Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12530/19540
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dc.contributor.authorPitarch, Aida
dc.contributor.authorNombela, César
dc.contributor.authorGil, Concha
dc.date.accessioned2019-06-28T12:32:25Z-
dc.date.available2019-06-28T12:32:25Z-
dc.date.issued2016-03
dc.identifier.citationData Brief.2016 Mar;(6):257-61
dc.identifier.issn2352-3409
dc.identifier.urihttps://hdl.handle.net/20.500.12530/19540-
dc.description.abstractThe characterization of pathogen-specific antigenic proteins at the protein species level is crucial in the development and molecular optimization of novel immunodiagnostics, vaccines or immunotherapeutics for infectious diseases. The major requirements to achieve this molecular level are to obtain 100% sequence coverage and identify all post-translational modifications of each antigenic protein species. In this article, we show nearly complete sequence information for five discrete antigenic species of Candida albicans Tdh3 (glyceraldehyde-3-phosphate dehydrogenase), which have been reported to be differentially recognized both among candidemia patients and between candidemia and control patients. A comprehensive description of the top-down immunoproteomic strategy used for seroprofiling at the C. albicans protein species level in candidemia as well as for the chemical characterization of this immunogenic protein (based on high-resolution 2-DE, Western blotting, peptide mass fingerprinting, tandem mass spectrometry and de novo peptide sequencing) is also provided. The top-down characterization data on the speciation of the C. albicans immunome in candidemia presented here are related to our research article entitled "Seroprofiling at the Candida albicans protein species level unveils an accurate molecular discriminator for candidemia" (Pitarch et al., J. Proteomics, 2015, http://dx.doi.org/10.1016/j.jprot.2015.10.022).
dc.language.isoeng
dc.rightsopenAccess-
dc.subjectBiomarkers
dc.subjectCandidemia
dc.subjectDiagnosis
dc.subjectGlyceraldehyde-3-phosphate dehydrogenase
dc.subjectImmunome
dc.subjectImmunoproteomics
dc.subjectInvasive candidiasis
dc.subjectProtein species
dc.subjectSerologic response
dc.subjectSerological proteome analysis
dc.titleTop-down characterization data on the speciation of the Candida albicans immunome in candidemia.
dc.typeArtículo
dc.identifier.pubmedID26862568
dc.format.volume6
dc.format.page257-61
dc.identifier.journalData in brief
dc.identifier.doi10.1016/j.dib.2015.11.054
dc.identifier.pmcPMC4707175
dc.pubmedtypeJournal Article
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. U. Ramón y Cajal > Artículos

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