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dc.contributor.authorTatjer, Laura
dc.contributor.authorSacristán-Reviriego, Almudena
dc.contributor.authorCasado, Carlos
dc.contributor.authorGonzález, Asier
dc.contributor.authorRodríguez-Porrata, Boris
dc.contributor.authorPalacios, Lorena
dc.contributor.authorCanadell, David
dc.contributor.authorSerra-Cardona, Albert
dc.contributor.authorMartín, Humberto
dc.contributor.authorMolina, María
dc.contributor.authorAriño, Joaquín
dc.identifier.citationGenetics.2016 Jan;(202)1:141-56
dc.description.abstractThe Saccharomyces cerevisiae type 2C protein phosphatase Ptc1 is required for a wide variety of cellular functions, although only a few cellular targets have been identified. A genetic screen in search of mutations in protein kinase-encoding genes able to suppress multiple phenotypic traits caused by the ptc1 deletion yielded a single gene, MKK1, coding for a MAPK kinase (MAPKK) known to activate the cell-wall integrity (CWI) Slt2 MAPK. In contrast, mutation of the MKK1 paralog, MKK2, had a less significant effect. Deletion of MKK1 abolished the increased phosphorylation of Slt2 induced by the absence of Ptc1 both under basal and CWI pathway stimulatory conditions. We demonstrate that Ptc1 acts at the level of the MAPKKs of the CWI pathway, but only the Mkk1 kinase activity is essential for ptc1 mutants to display high Slt2 activation. We also show that Ptc1 is able to dephosphorylate Mkk1 in vitro. Our results reveal the preeminent role of Mkk1 in signaling through the CWI pathway and strongly suggest that hyperactivation of Slt2 caused by upregulation of Mkk1 is at the basis of most of the phenotypic defects associated with lack of Ptc1 function.
dc.subjectSaccharomyces cerevisiae
dc.subjectcell-wall integrity pathway
dc.subjectprotein dephosphorylation
dc.subjectsynthetic genetic interactions
dc.subject.meshMitogen-Activated Protein Kinase Kinases
dc.subject.meshMitogen-Activated Protein Kinases
dc.subject.meshProtein Phosphatase 2
dc.subject.meshSaccharomyces cerevisiae
dc.subject.meshSaccharomyces cerevisiae Proteins
dc.subject.meshSignal Transduction
dc.titleWide-Ranging Effects of the Yeast Ptc1 Protein Phosphatase Acting Through the MAPK Kinase Mkk1.
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. U. Ramón y Cajal > Artículos

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