Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples.

Cura, Carolina I; Duffy, Tomas; Lucero, Raúl H; Bisio, Margarita; Péneau, Julie; Jimenez-Coello, Matilde; Calabuig, Eva; Gimenez, María J; Valencia Ayala, Edward; Kjos, Sonia A; Santalla, José; Mahaney, Susan M; Cayo, Nelly M; Nagel, Claudia; Barcán, Laura; Málaga Machaca, Edith S; Acosta Viana, Karla Y; Brutus, Laurent; Ocampo, Susana B; Aznar, Christine; Cuba Cuba, Cesar A; Gürtler, Ricardo E; Ramsey, Janine M; Ribeiro, Isabela; VandeBerg, John L; Yadon, Zaida E; Osuna, Antonio; Schijman, Alejandro G

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Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12530/21844

Title:	Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples.
Authors:	Cura, Carolina I Duffy, Tomas Lucero, Raúl H Bisio, Margarita Péneau, Julie Jimenez-Coello, Matilde Calabuig, Eva Gimenez, María J Valencia Ayala, Edward Kjos, Sonia A Santalla, José Mahaney, Susan M Cayo, Nelly M Nagel, Claudia Barcán, Laura Málaga Machaca, Edith S Acosta Viana, Karla Y Brutus, Laurent Ocampo, Susana B Aznar, Christine Cuba Cuba, Cesar A Gürtler, Ricardo E Ramsey, Janine M Ribeiro, Isabela VandeBerg, John L Yadon, Zaida E Osuna, Antonio Schijman, Alejandro G View more
Mesh:	Adolescent Adult Biological Assay Chagas Disease Child Child, Preschool Coinfection Female Genetic Variation Genotype Humans Male Molecular Typing Real-Time Polymerase Chain Reaction Sensitivity and Specificity Trypanosoma cruzi View more
Issue Date:	May-2015
Citation:	PLoS Negl Trop Dis.2015 May;(9)5:e0003765
Abstract:	Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI-TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivity and analytical sensitivity. Most of them can be only applied to cultured stocks. In this context, we aimed to develop a multiplex Real-Time PCR method to identify the six T. cruzi DTUs using TaqMan probes (MTq-PCR).
PMID:	25993316
URI:	https://hdl.handle.net/20.500.12530/21844
Rights:	openAccess
Appears in Collections:	Fundaciones e Institutos de Investigación > IIS H. U. La Paz > Artículos

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