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Title: 3D imaging in CUBIC-cleared mouse heart tissue: going deeper.
Keywords: (000.1430) Biology and medicine
(180.1790) Confocal microscopy
Issue Date: 1-Sep-2016
Citation: Biomed Opt Express.2016 Sep;(7)9:3716-3720
Abstract: The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.
PMID: 27699132
Rights: openAccess
ISSN: 2156-7085
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. General U. Gregorio Marañón > Artículos

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