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|Title:||Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse.|
|Citation:||Methods Mol. Biol..2017;(1584):31-49|
|Abstract:||The immune synapse (IS) is a specialized structure that enables cell-cell communication between immune cells. As such, it involves direct cell-to-cell contact. It is sustained by cytoskeletal components that allow the intracellular polarization of different organelles and the surface re-organization of signaling and adhesion receptors. The tubulin-based cytoskeleton is a key player in IS formation and signaling. We describe methods to analyze through Western blot and microscopy analysis the polarization to the IS of the centrosome, also known as microtubule-organizing center (MTOC), the dynamics of microtubule growth and polymerization from the MTOC to the IS and the activation of signaling molecules.|
|Appears in Collections:||Fundaciones e Institutos de Investigación > IIS H. U. La Princesa > Artículos|
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