Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12530/33330
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dc.contributor.authorGarcía-Romero, Noemí
dc.contributor.authorCarrión-Navarro, Josefa
dc.contributor.authorEsteban-Rubio, Susana
dc.contributor.authorLázaro-Ibáñez, Elisa
dc.contributor.authorPeris-Celda, María
dc.contributor.authorAlonso, Marta M
dc.contributor.authorGuzmán-De-Villoria, Juan
dc.contributor.authorFernández-Carballal, Carlos
dc.contributor.authorde Mendivil, Ana Ortiz
dc.contributor.authorGarcía-Duque, Sara
dc.contributor.authorEscobedo-Lucea, Carmen
dc.contributor.authorPrat-Acín, Ricardo
dc.contributor.authorBelda-Iniesta, Cristóbal
dc.contributor.authorAyuso-Sacido, Angel
dc.date.accessioned2019-06-28T17:17:54Z-
dc.date.available2019-06-28T17:17:54Z-
dc.date.issued2017-01-03
dc.identifier.citationOncotarget.2017 Jan;(8)1:1416-1428
dc.identifier.urihttps://hdl.handle.net/20.500.12530/33330-
dc.description.abstractTumor-cell-secreted extracellular vesicles (EVs) can cross the disrupted blood-brain barrier (BBB) into the bloodstream. However, in certain gliomas, the BBB remains intact, which might limit EVs release. To evaluate the ability of tumor-derived EVs to cross the BBB, we used an orthotopic xenotransplant mouse model of human glioma-cancer stem cells featuring an intact BBB. We demonstrated that all types of tumor cells-derived EVs-apoptotic bodies, shedding microvesicles and exosomes-cross the intact BBB and can be detected in the peripheral blood, which provides a minimally invasive method for their detection compared to liquid biopsies obtained from cerebrospinal fluid (CSF). Furthermore, these EVs can be readily distinguished from total murine EVs, since they carry human-specific DNA sequences relevant for GBM biology. In a small cohort of glioma patients, we finally demonstrated that peripheral blood EVs cargo can be successfully used to detect the presence of IDH1G395A, an essential biomarker in the current management of human glioma.
dc.language.isoeng
dc.rightsopenAccess-
dc.subjectbiomarkers
dc.subjectblood-brain barrier
dc.subjectbrain tumors
dc.subjectextracellular vesicles
dc.subject.meshAdult
dc.subject.meshAnimals
dc.subject.meshBase Sequence
dc.subject.meshBlood-Brain Barrier
dc.subject.meshBrain Neoplasms
dc.subject.meshDNA, Neoplasm
dc.subject.meshDisease Models, Animal
dc.subject.meshExtracellular Vesicles
dc.subject.meshFemale
dc.subject.meshGlioma
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Nude
dc.subject.meshMiddle Aged
dc.titleDNA sequences within glioma-derived extracellular vesicles can cross the intact blood-brain barrier and be detected in peripheral blood of patients.
dc.typeArtículo
dc.identifier.pubmedID27902458
dc.format.volume8
dc.format.page1416-1428
dc.identifier.e-issn1949-2553
dc.identifier.journalOncotarget
dc.identifier.doi10.18632/oncotarget.13635
dc.format.number1
dc.identifier.pmcPMC5352065
dc.pubmedtypeJournal Article
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. General U. Gregorio Marañón > Artículos
Fundaciones e Institutos de Investigación > IIS H. U. La Paz > Artículos

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