Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12530/38154
Title: In-depth resistome analysis by targeted metagenomics.
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Issue Date: 2018
Citation: Microbiome.2018 01;(6)1:11
Abstract: Antimicrobial resistance is a major global health challenge. Metagenomics allows analyzing the presence and dynamics of "resistomes" (the ensemble of genes encoding antimicrobial resistance in a given microbiome) in disparate microbial ecosystems. However, the low sensitivity and specificity of available metagenomic methods preclude the detection of minority populations (often present below their detection threshold) and/or the identification of allelic variants that differ in the resulting phenotype. Here, we describe a novel strategy that combines targeted metagenomics using last generation in-solution capture platforms, with novel bioinformatics tools to establish a standardized framework that allows both quantitative and qualitative analyses of resistomes.
PMID: 29335005
URI: https://hdl.handle.net/20.500.12530/38154
Rights: openAccess
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. U. Ramón y Cajal > Artículos

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