Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12530/41479
Title: Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase.
Authors: 
Keywords: 
Mesh: 
Issue Date: 2017
Citation: Proc. Natl. Acad. Sci. U.S.A..2017 10;(114)43:11291-11296
Abstract: ATP synthase is a rotating membrane protein that synthesizes ATP through proton-pumping activity across the membrane. To unveil the mechanical impact of this molecular active pump on the bending properties of its lipid environment, we have functionally reconstituted the ATP synthase in giant unilamellar vesicles and tracked the membrane fluctuations by means of flickering spectroscopy. We find that ATP synthase rotates at a frequency of about 20 Hz, promoting large nonequilibrium deformations at discrete hot spots in lipid vesicles and thus inducing an overall membrane softening. The enhanced nonequilibrium fluctuations are compatible with an accumulation of active proteins at highly curved membrane sites through a curvature-protein coupling mechanism that supports the emergence of collective effects of rotating ATP synthases in lipid membranes.
PMID: 29073046
URI: https://hdl.handle.net/20.500.12530/41479
Rights: openAccess
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. U. 12 de Octubre > Artículos

Files in This Item:
File Description SizeFormat 
PMC5664490.pdf1.14 MBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.