Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12530/43838
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dc.contributor.authorMandriani, Barbara
dc.contributor.authorCastellana, Stefano
dc.contributor.authorRinaldi, Carmela
dc.contributor.authorManzoni, Marta
dc.contributor.authorVenuto, Santina
dc.contributor.authorRodriguez-Aznar, Eva
dc.contributor.authorGalceran, Juan
dc.contributor.authorNieto, M Angela
dc.contributor.authorBorsani, Giuseppe
dc.contributor.authorMonti, Eugenio
dc.contributor.authorMazza, Tommaso
dc.contributor.authorMerla, Giuseppe
dc.contributor.authorMicale, Lucia
dc.date.accessioned2019-08-30T09:01:40Z-
dc.date.available2019-08-30T09:01:40Z-
dc.date.issued2016
dc.identifier.citationSci Rep.2016 09;(6):32474
dc.identifier.urihttps://hdl.handle.net/20.500.12530/43838-
dc.description.abstractTo orchestrate the genomic response to cellular stress signals, p53 recognizes and binds to DNA containing specific and well-characterized p53-responsive elements (REs). Differences in RE sequences can strongly affect the p53 transactivation capacity and occur even between closely related species. Therefore, the identification and characterization of a species-specific p53 Binding sistes (BS) consensus sequence and of the associated target genes may help to provide new insights into the evolution of the p53 regulatory networks across different species. Although p53 functions were studied in a wide range of species, little is known about the p53-mediated transcriptional signature in Danio rerio. Here, we designed and biochemically validated a computational approach to identify novel p53 target genes in Danio rerio genome. Screening all the Danio rerio genome by pattern-matching-based analysis, we found p53 RE-like patterns proximal to 979 annotated Danio rerio genes. Prioritization analysis identified a subset of 134 candidate pattern-related genes, 31 of which have been investigated in further biochemical assays. Our study identified runx1, axin1, traf4a, hspa8, col4a5, necab2, and dnajc9 genes as novel direct p53 targets and 12 additional p53-controlled genes in Danio rerio genome. The proposed combinatorial approach resulted to be highly sensitive and robust for identifying new p53 target genes also in additional animal species.
dc.language.isoeng
dc.rightsopenAccess-
dc.subject.meshAnimals
dc.subject.meshAxin Protein
dc.subject.meshBase Sequence
dc.subject.meshBinding Sites
dc.subject.meshCalcium-Binding Proteins
dc.subject.meshCollagen Type IV
dc.subject.meshCore Binding Factor Alpha 2 Subunit
dc.subject.meshEvolution, Molecular
dc.subject.meshGene Expression Regulation
dc.subject.meshHSC70 Heat-Shock Proteins
dc.subject.meshHSP40 Heat-Shock Proteins
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshProtein Binding
dc.subject.meshSequence Alignment
dc.subject.meshSignal Transduction
dc.subject.meshTNF Receptor-Associated Factor 4
dc.subject.meshTumor Suppressor Protein p53
dc.subject.meshZebrafish
dc.subject.meshZebrafish Proteins
dc.subject.meshGenome
dc.subject.meshResponse Elements
dc.subject.meshTranscription, Genetic
dc.titleIdentification of p53-target genes in Danio rerio.
dc.typeArtículo
dc.identifier.pubmedID27581768
dc.format.volume6
dc.format.page32474
dc.identifier.e-issn2045-2322
dc.identifier.journalScientific reports
dc.identifier.doi10.1038/srep32474
dc.identifier.pmcPMC5007497
dc.pubmedtypeJournal Article
dc.pubmedtypeResearch Support, Non-U.S. Gov't
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. U. Ramón y Cajal > Artículos

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