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Title: Prevalence, detection and characterisation of fosfomycin-resistant Escherichia coli strains carrying fosA genes in Community of Madrid, Spain.
[Loras, Cristina; Alos, Juan-Ignacio] Hosp Univ Getafe, Serv Microbiol, Carretera Toledo Km 12,500, Madrid 28905, Spain.
[Gonzalez-Prieto, Andrea] Hosp Infanta Sofia, Lab Cent BR Salud, Madrid, Spain.
[Perez-Vazquez, Maria; Bautista, Veronica; avila, Alicia; Campoy, Pedro Sola; Oteo-Iglesias, Jesus] Inst Salud Carlos III, Ctr Nacl Microbiol, Lab Referencia & Invest Resistencia & Antibiot &, Madrid, Spain.
[Perez-Vazquez, Maria; Bautista, Veronica; Oteo-Iglesias, Jesus] Red Espanola Invest Patol Infecciosa REIPI, Madrid, Spain.
Issue Date: 21-Mar-2021
Citation: J Glob Antimicrob Resist. 2021;25:137-141
Abstract: The aim of this this study was to describe the presence of different variants of the fosA gene in fosfomycin-resistant Escherichia coli strains in Madrid, Spain. fos genes were searched for in 55 E. coli strains collected from seven representative hospitals located in Madrid. A phenotypic screening test was performed following the disk diffusion method with sodium phosphonoformate added as described by Nakamura et al. Additionally, a molecular study based on PCR was used to confirm the screening results. Positive strains for fos genes were further subjected to whole-genome sequencing (WGS). Phenotypic screening was positive in 9/55 strains (16.4%), although genotypic detection was positive in only 3 (fosA3, fosA4 and fosA6). Thus, the prevalence of fos genes in Madrid was 5.5% (3/55). WGS data were not available for the fosA6-positive strain. One isolate with fosA3 (ST69) carried a blaCTX-M-55 gene and seven virulence genes (air, eilA, iha, iss, lpfA, sat and senB). The fosA4-positive isolate (ST4038) carried the virulence genes iss, lpfA, iroN and mchF. Both fos genes were located between two IS26 mobile elements of a plasmid. We detected the presence of different variants of plasmid-mediated fosA genes in fosfomycin-resistant E. coli strains in Madrid, Spain. Despite the few reports in Europe, it would be of interest to monitor the spread of these acquired resistance genes.
PMID: 33757820
Rights: openAccess
Appears in Collections:Fundaciones e Institutos de Investigación > IIS H. U. Getafe > Artículos
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